Journal: Journal of Virology
Article Title: Immunization with DNA Plasmids Coding for Crimean-Congo Hemorrhagic Fever Virus Capsid and Envelope Proteins and/or Virus-Like Particles Induces Protection and Survival in Challenged Mice
doi: 10.1128/JVI.02076-16
Figure Lengend Snippet: Serum IgG subclass reactivity for each pooled A129 IFNAR −/− serum sample to CCHFV antigen. In the top panel, the subclass pattern of the serum pools (indicated on the x axis) is shown prior to CCHFV challenge, and in the bottom panel the serum IgG subclass pattern is shown at 10 days post-CCHFV challenge (at day of sacrifice).
Article Snippet: An inactivated CCHFV antigen was used for coating at a 1:300 dilution in 0.05 M Na 2 CO 3 -NaHCO 3 buffer, pH 9.6, in flat-bottomed 96-well plates (PolySorp, Nunc, Thermo Scientific).
Techniques: